|
KMID : 0358420070500040601
|
|
Korean Journal of Obstetrics and Gynecology
2007 Volume.50 No. 4 p.601 ~ p.611
|
|
|
Association of genetic polymorphisms in the peroxisome proliferator-activated receptor-γ and methylenetetrahydrofolate reductase with preeclampsia in Korean women
|
|
Lee So-Hyun
Park Bo-Hyun
Park Mi-Hye
Park Hye-Sook
Chun Sun-Hee
Ahn Jung-Ja
Ryu Hyun-Mee
Lee Kwang-Soo
Park Hyun-Young
Kim Young-Ju
|
|
|
Abstract
|
|
|
Objective: To investigate whether polymorphisms of genes encoding peroxisome proliferator-activated receptor-γ (PPARγ) and methylenetetrahydrofolate reductase (MTHFR) are associated with preeclmapsia in Korean women and also to demonstrate whether there is any haplotypic association between preeclampsia and those genes.
Methods: DNA was extracted from whole blood of 226 preeclampsia patients and 235 healthy pregnant women. The genotypes of SNPs in PPARγ (-796A>G, P12A (C>G), H447H (161C>T)) and MTHFR (A222V (677C>T), E429A (1298A>C), R594Q (1793G>A)) were analyzed by a single base primer extension assay using a SNaPShot assay kit. Results were analyzed with the Student’s t-test, Chi-square test, and Logistic regression analysis. Haplotype analyses were performed using Haploview 3.2 version.
Results: There were no significant differences in genotype or allele frequencies of PPARγ and MTHFR gene polymorphisms between preeclampsia patients and controls (p>0.05). No increase in the risk of preeclampsia for those genes was observed under any model of inheritance. Among SNPs of the PPARγ, MTHFR genes, only SNPs in MTHFR gene (677C>T, 1298A>C, 1793G>A) were in a strong linkage disequilibrium with each other (Lod score>2.0), but there were no significant differences in genotype distribution of haplotypes of MTHFR gene (TAG, CAG, CCA, CCG) between preeclampsia patients and controls (p>0.05). No statistically significant associations were observed between any haplotypes of MTHFR gene and preeclampsia risk.
Conclusion: This study suggest that SNPs in PPARγ and MTHFR gene were not associated with preeclampsia in Korean women, and its haplotypes were also not associated with preeclampsia.
|
|
|
KEYWORD
|
|
|
Preeclampsia, Single nucleotide polymorphism, PPARγ, MTHFR, Haplotypes
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|
|